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qRTPCR

We develop and test custom qRTPCR assays using both Life Technologies Taqman and Roche Light Cycler chemistries for quantitative measurement of:

  • Gene expression
  • miRNA
  • Copy number
  • Single nucleotide polymorphisms
  • Mutations

Assays can be run under both non-GLP and GLP in our laboratory. In all cases, we ensure robustness and confidence in your data by validating each assay against the following parameters:

  • Sensitivity
  • Specificity
  • Linear range, dynamic range
  • Limit of quantitation, limit of detection
  • Precision/reproducibility
  • Accuracy

Our qRTPCR platforms are manufactured under design control, meaning that they are “regulatory-ready”, providing a complete path toward diagnostic development.
SAMPLE TYPES qRTPCR methods are validated in-house for the following sample types:

  • Cell pellets
  • Frozen tissue
  • Formalin-fixed paraffin embedded (FFPE) tissue
  • Whole blood
  • Serum/Plasma
  • Urine

Please contact us for sample collection and processing SOPs, to ensure that your precious tissue is best maintained for qRTPCR analysis.

DATA ANALYSIS

We report data back to the customer in the following formats:

  • RawCT
  • ΔCT (target gene normalized to reference gene(s))
  • ΔΔCT (comparison of one ΔCT to another ΔCT)
    • Example: comparison of gene expression between a matched tumor/normal
  • Visualizations/plots of differentially expressed genes